Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1976 Sep;58(3):426–432. doi: 10.1104/pp.58.3.426

Citric Acid Cycle Activity in Mitochondria Isolated from Mung Bean Hypocotyls 1,2

Emma J Bowman a,3, Hiroshi Ikuma a,4, Howard J Stein a,5
PMCID: PMC542260  PMID: 16659692

Abstract

Citric acid cycle activity in mitochondria from mung bean (Phaseolus aureus var. Jumbo) hypocotyls were examined by surveying (a) characteristics of oxidation of cycle intermediates; (b) activities of cycle enzymes in mitochondrial extracts; (c) contents of cycle intermediates and electron transport components in isolated mitochondria; and (d) time-course changes of products formed during oxidation of succinate, malate, and citrate. Isolated mitochondria are deficient in thiamine pyro-phosphate and somewhat so in adenylates, but apparently sufficient in CoA, NAD, and electron transport carriers. Cycle activity in the mitochondria is not directly correlated with the activities of the enzymes measured in extracts. These studies led to the conclusion that the region between malate and citrate is an important regulatory area in citric acid cycle functioning in isolated mung bean mitochondria.

Full text

PDF
426

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashwell M., Work T. S. The biogenesis of mitochondria. Annu Rev Biochem. 1970;39:251–290. doi: 10.1146/annurev.bi.39.070170.001343. [DOI] [PubMed] [Google Scholar]
  2. Avron M., Biale J. B. Metabolic Processes in Cytoplasmic Particles of the Avocado Fruit. III. The Operation of the Tricarboxylic Acid Cycle. Plant Physiol. 1957 Mar;32(2):100–105. doi: 10.1104/pp.32.2.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barbareschi D., Longo G. P., Servettaz O., Zulian T., Longo C. P. Citrate synthetase in mitochondria and glyoxysomes of maize scutellum. Plant Physiol. 1974 Jun;53(6):802–807. doi: 10.1104/pp.53.6.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bowman E. J., Ikuma H. Regulation of Malate Oxidation in Isolated Mung Bean Mitochondria: II. Role of Adenylates. Plant Physiol. 1976 Sep;58(3):438–446. doi: 10.1104/pp.58.3.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bowman E. J., Ikuma H. Regulation of malate oxidation in isolated mung bean mitochondria: I. Effects of oxaloacetate, pyruvate, and thiamine pyrophosphate. Plant Physiol. 1976 Sep;58(3):433–437. doi: 10.1104/pp.58.3.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CHANCE B., WILLIAMS G. R. The respiratory chain and oxidative phosphorylation. Adv Enzymol Relat Subj Biochem. 1956;17:65–134. doi: 10.1002/9780470122624.ch2. [DOI] [PubMed] [Google Scholar]
  7. Coleman J. O., Palmer J. M. The oxidation of malate by isolated plant mitochondria. Eur J Biochem. 1972 Apr 24;26(4):499–509. doi: 10.1111/j.1432-1033.1972.tb01792.x. [DOI] [PubMed] [Google Scholar]
  8. Douce R., Bonner W. D., Jr Oxalacetate control of Krebs cycle oxidations in purified plant mitochondria. Biochem Biophys Res Commun. 1972 May 12;47(3):619–624. doi: 10.1016/0006-291x(72)90923-0. [DOI] [PubMed] [Google Scholar]
  9. Douce R., Christensen E. L., Bonner W. D., Jr Preparation of intaintact plant mitochondria. Biochim Biophys Acta. 1972 Aug 17;275(2):148–160. doi: 10.1016/0005-2728(72)90035-7. [DOI] [PubMed] [Google Scholar]
  10. ESTABROOK R. W., MAITRA P. K. A fluorimetric method for the quantitative microanalysis of adenine and pyridine nucleotides. Anal Biochem. 1962 May;3:369–382. doi: 10.1016/0003-2697(62)90065-9. [DOI] [PubMed] [Google Scholar]
  11. Ikuma H. Necessary conditions for isolation of tightly coupled higher plant mitochondria. Plant Physiol. 1970 Jun;45(6):773–781. doi: 10.1104/pp.45.6.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. LaNoue K. F., Bryla J., Williamson J. R. Feedback interactions in the control of citric acid cycle activity in rat heart mitochondria. J Biol Chem. 1972 Feb 10;247(3):667–679. [PubMed] [Google Scholar]
  14. LaNoue K., Nicklas W. J., Williamson J. R. Control of citric acid cycle activity in rat heart mitochondria. J Biol Chem. 1970 Jan 10;245(1):102–111. [PubMed] [Google Scholar]
  15. Lance C., Bonner W. D. The respiratory chain components of higher plant mitochondria. Plant Physiol. 1968 May;43(5):756–766. doi: 10.1104/pp.43.5.756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lance C., Hobson G. E., Young R. E., Biale J. B. Metabolic processes in cytoplasmic particles of the avocado fruit. IX. The oxidation of pyruvate and malate during the climacteric cycle. Plant Physiol. 1967 Apr;42(4):471–478. doi: 10.1104/pp.42.4.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Macrae A. R., Moorhouse R. The oxidation of malate by mitochondria isolated from cauliflower buds. Eur J Biochem. 1970 Sep;16(1):96–102. doi: 10.1111/j.1432-1033.1970.tb01058.x. [DOI] [PubMed] [Google Scholar]
  18. Oestreicher G., Hogue P., Singer T. P. Regulation of Succinate Dehydrogenase in Higher Plants: II. Activation by Substrates, Reduced Coenzyme Q, Nucleotides, and Anions. Plant Physiol. 1973 Dec;52(6):622–626. doi: 10.1104/pp.52.6.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. RACKER E. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim Biophys Acta. 1950 Jan;4(1-3):211–214. doi: 10.1016/0006-3002(50)90026-6. [DOI] [PubMed] [Google Scholar]
  20. Steer B. T., Beevers H. Compartmentation of Organic Acids in Corn Roots. III. Utilization of Exogenously Supplied Acids. Plant Physiol. 1967 Sep;42(9):1197–1201. doi: 10.1104/pp.42.9.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wedding R. T., Black M. K. Nucleotide activation of cauliflower alpha-ketoglutarate dehydrogenase. J Biol Chem. 1971 Mar 25;246(6):1638–1643. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES