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. 2017 Mar;13(1):35–42.

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© Juan Rebollo et al. Licensee Master Publishing Group

This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ESM-1 and CXC chemokine expression of siRNA transfected PC-3 cells. A, At 48 h post transfection, transcript level of ESM-1 in cells transfected with ESM-1 siRNA was significantly reduced compared with cells transfected with control siRNA. Knockdown of ESM-1 in PC-3 cells did not affect the transcript expression of most CXC chemokines, except for CXCL3. ESM-1 silencing was associated to decreased transcript levels of CXCL3 chemokine, compared to cells transfected with control siRNA or untransfected cells; B, Knockdown of ESM-1 in PC-3 cells significantly reduced the ESM-1 protein level and it was associated to decreased level of CXCL3. ESM-1 knockdown did not affect the protein expression of the remaining CXC chemokines evaluated. Chemokines evaluated were CXCL1/GROα, CXCL3, CXCL8/IL8, CXCL9/MIG, CXCL10/ IP-10, and CXCL11/I-TAC. ESM-1 and CXC chemokine protein levels in cell supernatants are shown as mean ± SEM. siControl: PC-3 cells transfected with control siRNA.siESM-1: PC-3 cells transfected with ESM-1 siRNA for 48 h.