Fig. 1.
Cell density-dependent response to gemcitabine in pancreatic cancer. (A) A table illustrating YAP nuclear localization and TEAD transcriptional activity in low- and high-cell density conditions. (B) A schematic showing live-cell kinetic cell growth assay used to characterize the phenotypic effect of gemcitabine in a panel of pancreatic cancer cell lines. Gemcitabine-mediated GC50 (50% inhibition in growth compared with control) for each cell line was calculated. (C) A plot showing the effect on gemcitabine on growth of 15 pancreatic cancer cell lines. Literature-curated values of cell line-specific GC50 are also indicated. See SI Appendix, Table S1 for details. (D) Cell density affects gemcitabine response. Plots show cell growth curves of Aspc1 (Top) and Patu-8988S (Bottom) cells grown in low- or high-density conditions. (E) All cell lines tested were sensitive or resistant to gemcitabine in low- or high-density conditions, respectively. (F) Replating cells at low density restored sensitive to gemcitabine.