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. 2017 Apr 17;114(18):E3642–E3651. doi: 10.1073/pnas.1619266114

Fig. 3.

Fig. 3.

Loss of drebrin leads to enhanced endocytosis of dynamin-dependent cargo. (A) WT and DBN1 KO HEK293 cells were incubated with FITC-conjugated transferrin (Tfn, 20 μg/mL) and fixed at indicated time points for confocal microscopy to examine intracellular transferrin (green) and nucleus (DAPI, blue). (Scale bar in panels and single z slices, 20 μm.) (B) WT and DBN1 KO HEK293 cells were incubated with FITC-conjugated cholera toxin subunit B (CTxB, 10 μg/mL) and fixed at indicated time points for confocal microscopy for internalized toxin (green) and nucleus (DAPI, blue). (Scale bar in panels and single z slices, 20 μm.) (C) WT and DBN1 KO HEK293 cells were infected with human adenovirus serotype 5 (HAdV5) or simian vacuolating virus 40 (SV40) at MOI = 0.1 for 1 h. Expression levels of hexon (HAdV5) and large T antigen (SV40) were measured by RT-qPCR and normalized to that of GAPDH. For all figures, experiments were repeated at least three times. Data are represented as mean ± SEM. Statistical significance is determined by Student’s t test (***P ≤ 0.001).