Fig. 7.

qRT-PCR analysis of gene expression of WD response proteins in C. humilis. RNA was extracted from WD treatment for 0, 12, and 24 h days, respectively. Values represent the mean of three biological replicates and two technical replicates. Each data point represents mean ± SE (n = 3). * and ** indicate values that differ significantly from controls at p ≤ 0.05 and p ≤ 0.01, respectively, according to LSD test. CA, carbonic anhydrase; Rpi, Ribose 5-phosphate isomerase; DHAR, dehydroascorbate reductase; GPX, glutathione peroxidase; TIM, triosephosphate isomerase; ME, malic enzyme; TK, transketolase; RRM, RNA recognition motif; HSP70, heat-shock protein 70; Echi, acidic endochitinase