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. 2017 May 8;36:64. doi: 10.1186/s13046-017-0537-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — High expression of HSF1 in pancreatic cancer tissues and association with poor prognosis in patients. a Representative image of immunohistochemical detection of the expression of HSF1 and Ki-67 proteins in 50 pairs of pancreatic cancer and para-carcinoma tissue specimens. Scale bar, 50 μm. b Semi-quantitative analysis of HSF1 protein expression in pancreatic cancer and para-carcinoma tissues. Differences were analyzed by paired t-test and data represent the mean ± standard deviation of three independent experiments. ***P < 0.001. c Correlation between HSF1 and the proliferation marker, Ki-67, in pancreatic cancer tissues by immunohistochemistry. Figure shows the Ki-67 index in pancreatic cancer tissues with low or high expression of HSF1. d and e Receiver operating characteristic (ROC) curve analysis of the correlation between the expression of HSF1 protein in patients with pancreatic cancer and overall survival (d) and recurrence-free survival (e). f and g Kaplan–Meier survival curve analysis of the correlation between the expression of HSF1 protein in patients with pancreatic cancer and overall survival (f) and recurrence-free survival (g)