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. 2017 May 8;36:64. doi: 10.1186/s13046-017-0537-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Detection of HSF1 expression in different pancreatic cancer cell lines and HSF1-siRNA screening verification. a and b Western blot (a) and RT-PCR (b) were used to detect the expression levels of HSF1 in four pancreatic cancer cell lines (ASPC-1, BXPC -3, CFPAC-1 and PANC-1). c and d Detection of HSF1 expression in PANC-1 cells after transfection with HSF1-siRNA#1 and HSF1-siRNA#2 by Western blot (c) and RT-PCR (d). Differences were analyzed by paired t-test and data represent the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001