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SMAC is a mediator of mitochondrial apoptosis in HSF1-regulated pancreatic cancer cells. a and b Annexin V/PI double staining was used to detect PANC-1 cell apoptosis. c CCK-8 assay of PANC-1 cell proliferation. d and e Western blot analysis of the expression of SMAC, XIAP, cleaved caspase 3 and cleaved caspase 9 proteins in PANC-1 cells. Differences were analyzed by paired t-test and data represent the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01
