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. 2017 May 8;10:24. doi: 10.1186/s13072-017-0129-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — CRISPR-dCas9-based epigenome editing for primary T cells. a A retroviral vector for the expression of dCas9-epigenome regulator fusion proteins from Moloney murine leukemia virus promoter long terminal repeats (ΔLTRs) and green fluorescent protein (GFP) from an internal ribosomal entry site (IRES). Retroviral and lentiviral vector for bicistronic expression of the gRNA from a U6 promoter (U6) and DsRed from a short EF1a promoter (EFS). b Protein expression of dCas9-epigenome regulator fusion proteins in transfected HEK293T cells was detected by western blot against anti-Flag antibody. Anti α-tubulin antibody was used for loading control