Figure 3. Activation of β1- and β2-AMPK complexes by direct small-molecule activators.

AMPKβ1 or AMPKβ2 expressing stable cell lines were generated in the β1β2 dKO HEK293T cells. Cells were incubated with different concentrations of 991 (A) or PF249 (B). Levels of ACC and Thr¹⁷² phosphorylation, together with β-subunit expression, were determined.