Figure 5.

MV formation does not require cytotoxicity. Cy5 conjugated SLO C530A was partially inactivated by freeze–thaw (SLO FT), fully inactivated by heat treatment at 37 °C for 15 min (SLO HT) or left unperturbed (SLO WT). (a) Cells were resuspended in RC with 20 μg/ml PI and challenged with equivalent masses of all three toxin subsets. (b) MV were isolated as described in Figure 3 from cells challenged with 250 HU/ml SLO WT or equivalent masses of other toxins. Graphs display the average±S.E.M., whereas blots show one representative experiment, of at least three independent experiments. *P<0.05, **P<0.01 and ***P<0.001