Figure 2.

Overexpression of mitochondrial SK2 channels attenuates mitochondrial respiration. (a) Representative oxygen slope of isolated mitochondria from HT22 cells. Left panel: definition of mitochondrial states (1, 2, 3, 4, 4u) by addition of indicated substances in a representative measurement. Right panel: corrected oxygen slope showing mitochondrial states after treatment with vehicle (VEH) or CyPPA (CY). Mitochondrial states are indicated as (2–4u). (b–e) Quantification of state 3 (left panels) and state 4u (right panels) of mitochondria pre-treated with vehicle (VEH) or CyPPA (CY) (b), of HT22 cells overexpressing GFP or SK2 (c), mitoGFP or mitoSK2 (d), or SK2 or mitoSK2 (e). Data are shown as mean±SD, n=3–5, *P<0.05, **P<0.01 compared to indicated control