Figure 3.

Targeted overexpression of SK2 channels in the mitochondria augments CyPPA-mediated preservation of mitochondrial integrity. Mitochondrial integrity was analyzed by means of fluorescent-activated cell sorting using fluorescent probes for mitochondrial membrane potential (a-d) or mitochondrial ROS formation (e-h) following glutamate exposure (16 h) in the presence or absence of CyPPA (CY) in wildtype HT22 cells (a, e), and HT22 cells overexpressing (b, f) GFP versus SK2, (c, g) mitoGFP versus mitoSK2, and (d, h) SK2 versus mitoSK2. Data are presented as mean±SD, n=3, *P<0.05, **P>0.01, ***P<0.001