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. 2017 Mar 24;24(5):889–902. doi: 10.1038/cdd.2017.34

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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p53 is a mediator of FAK silencing effects on EZH2 in HepG2 cells. (a) Representative WB and densitometric quantification for p53 in Control and shFAK HepG2 cells. GAPDH is reported as a loading control. Values are mean±SD of at least three independent experiments (**P<0.01; n=3). (b) Representative IF of serine 15 phosphorylated form of p53 in Control and shFAK HepG2 cells. DRAQ5 was used to stain nuclei ( × 60 magnification). (c) ChIP assays for p53 at the EZH2 promoter in Control and shFAK HepG2 cells. Values are expressed as percentage of input DNA of three independent experiments. IgG was used as negative ChIP control. (d) Relative mRNA expression of EZH2 gene as measured by qRT-PCR in Control and shFAK HepG2 cells after transient silencing with siC or sip53. Values are expressed as fold mean±SD (***P<0.001; n=3). (e) Relative mRNA expression of TAp53 gene as measured by qRT-PCR in Control and shFAK HepG2 cells after transient silencing with siC or sip53. Values are expressed as fold mean±SD (***P<0.001; n=3)