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. 2017 Mar 24;24(5):889–902. doi: 10.1038/cdd.2017.34

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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FAK silencing in HCC cells affects E2F2 and E2F3 binding to EZH2 promoter. ChIP assay of E2F2 (a) and E2F3 (b) on the EZH2 promoter in Control and shFAK HepG2 cells. ChIP assays of E2F2 (c) and E2F3 (d) on the EZH2 promoter in Control and shFAK Huh7.5 cells. Values are expressed as percentage of input DNA of three independent experiments. IgG was used as negative ChIP control. (e) Representative WB for pRb in Control and shFAK HepG2 and Huh7.5 cells (n=3). (f) Representative WB for phosphorylated cdks (p-cdks) in Control and shFAK HepG2 and Huh7.5 cells. α-tubulin is reported as a loading control (n=3)