Figure 4.

GFI1 controls an inflammatory signalling circuit that enhances invasive and migratory behaviour of human CRC Cells. (a) qRT-PCR of indicated cytokine mRNA in HT29 cells incubated with control medium or LSMCM for 24 h. (b) TGFβ concentrations by ELISA assays in supernatants from HT29 cells that were treated with LSMCM for the indicated periods. (c) qRT-PCR of shown cytokine mRNA in HT29 cells treated with LSMCM or LSMCM with the indicated shRNAs, siRNAs, or GFI1-expressing plasmids. (d) Schematic overview of the inflammation and TGFβ-induced regulatory circuit that controls CRC cell metastatic behaviour. (e) qRT-PCR of E-cadherin, Fibronectin, and Vimentin mRNA in HT29 cells transfected with GFI1-encoding plasmid or control. (f) Regulation of HT29 invasive and migratory behaviour by ectopically expressed GFI1. (g) Regulation of HT29 invasive and migratory behaviour by knocking down of GFI1 expression. (h) qRT-PCR of E-cadherin, Fibronectin and Vimentin mRNA in HT29 cells transfected with GFI1 shRNA or control. (a–c), (e–h) are shown as means±S.D. of three independent experiments. Statistically significant differences are indicated. *P<0.05; **P<0.01