Figure 6.

Gfi1 inhibits MC38 metastasis to liver. (a) Migratory and invasive activity of MC38 cells transfected with control or Gfi1-activation plasmids. (b) Immunoblot analysis of MC38-con and MC38-Gfi1 cells demonstrating effects of Gfi1 on indicated signalling proteins. (c) Liver metastases in mice injected intrasplenicly (i.s.) with MC38-con or MC38-Gfi1 cells. Arrows pointed to metastases in the liver. (d) Representative H&E-stained sections of the livers of mice injected with MC38-Gfi1 or MC38-con cells. (e) Weights of livers in mice injected with MC38-Gfi1 or MC38-con cells 16 days after i.s. injection. (f) Weight of mice injected with MC38-Gfi1 or MC38-con cells 16 days after i.s. injection. (g) Kaplan–Meier survival curve in respective MC38-Gfi1 and -con mice, log-rank test, P<0.0001. (h) GFI1 expression levels in the primary tumour of human CRC in patients who did (M1) or did not (M0) develop distal metastases in a TCGA data set