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. 2016 Sep 16;10(10):1516–1531. doi: 10.1016/j.molonc.2016.08.005

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© 2016 Federation of European Biochemical Societies

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graphic file with name MOL2-10-1516-g006.jpg — Autophagy inhibition on PD‐L1 expression and clinicopathological correlation with tumor progression. (A–B) The protein and mRNA expression level of PD‐L1 was assayed by western blot and RT‐PCR, respectively. (C–D) LC/LC‐DR cells were treated with autophagy inhibitor chloroquine (20 μΜ) or carboplatin (25 μΜ) for 24 h and PD‐L1 expression was measured by immunofluorescence analysis (magnification ×200). (F–G) Immunohistochemical analysis was performed in lung biopsies following chloroquine and carboplatin treatment (50 μΜ) for 1 h using mouse IgG1 isotype as negative control. Images were obtained through a Carl Zeiss microscope using image analysis software (Scale bar, 50 μm) (magnification ×400). (D) Quantification of immunohistochemistry by the intensity of the positive immunosignals in the tissue sections are showed in histograms (*P < 0.05, **P < 0.01). Stained tumor cells are shown at a final magnification (×200). Data represent the mean ± SD of three independent experiments. (*P < 0.05; **P < 0.01).

graphic file with name MOL2-10-1516-g007.jpg — Autophagy inhibition on PD‐L1 expression and clinicopathological correlation with tumor progression. (A–B) The protein and mRNA expression level of PD‐L1 was assayed by western blot and RT‐PCR, respectively. (C–D) LC/LC‐DR cells were treated with autophagy inhibitor chloroquine (20 μΜ) or carboplatin (25 μΜ) for 24 h and PD‐L1 expression was measured by immunofluorescence analysis (magnification ×200). (F–G) Immunohistochemical analysis was performed in lung biopsies following chloroquine and carboplatin treatment (50 μΜ) for 1 h using mouse IgG1 isotype as negative control. Images were obtained through a Carl Zeiss microscope using image analysis software (Scale bar, 50 μm) (magnification ×400). (D) Quantification of immunohistochemistry by the intensity of the positive immunosignals in the tissue sections are showed in histograms (*P < 0.05, **P < 0.01). Stained tumor cells are shown at a final magnification (×200). Data represent the mean ± SD of three independent experiments. (*P < 0.05; **P < 0.01).