Figure 5.
Inhibition of AGR2 increases p38 MAPK activity. A549 (A) and MCF‐7 cells (B) were transfected either with control or AGR2 specific siRNA and then exposed to cisplatin or doxorubicin. Changes in p38 MAPK activity directly correspond to fold changes in degree of phosphorylation of ATF‐2 fusion protein. Relative fold changes in amounts of phosphorylated ATF‐2 fusion protein as well as p38MAPK are indicated under corresponding bands. These numbers were determined after normalization to ACTB in relation to untreated cells with unaffected endogenous AGR2 expression. This is a representative figure of two independent determinations of p38 MAPK expression and activity.