Skip to main content
. 2016 Mar 25;10(6):910–920. doi: 10.1016/j.molonc.2016.03.003

Figure 1.

Figure 1

Overall strategy of phosphotyrosine peptide enrichment and LC‐MS/MS analysis. The experimental workflow for labeling of C. elegans, phosphotyrosine enrichment and LC‐MS/MS is shown. First, E. coli cultures were grown with SILAC isotopes and then fed to C. elegans over several generations until the worms were fully labeled. Following label incorporation, wild type and mutant samples were lysed and an equal protein amount of each labeled lysate was mixed together. Following mixing, one portion of the sample was subjected to whole proteome LC‐MS/MS analysis and the remaining portion was subjected to phosphotyrosine peptide enrichment and LC‐MS/MS analysis.