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. 2016 Mar 22;10(6):895–909. doi: 10.1016/j.molonc.2016.03.001

Figure 4.

Figure 4

miR‐424‐5p promotes invasion and migration through SOCS2. SCC‐15 cells were transfected with miR‐424‐5p mimics or scramble control (NC) for 24 h and then transfected with SOCS2 expression vector (without 3′‐UTR) or control vector for another 24 h. A: Western blot analysis of the SOCS2, STAT5 and phosphor‐STAT5. α‐Tubulin was used as protein loading control. Numerical values for protein band intensities are shown below the gels. The values were quantitated by densitometry and normalized to α‐tubulin. B: RT‐PCR analysis of SOCS2, MMP‐2 and MMP‐9. GAPDH was used as an internal control. C: Migration assay and D: Invasion assay using Boyden Chamber system. All data are presented as mean ± SE; ***, p < 0.001, one‐way ANOVA p < 0.0001.