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. 2016 Feb 23;10(6):838–849. doi: 10.1016/j.molonc.2016.02.002

Figure 3.

Figure 3

Tensin1 is a direct target of miR‐548j. (A) The endogenous expression of Tensin1 was examined in breast cancer cell lines using quantitative RT‐PCR. The values were normalized to GAPDH. (B) A description of two predicted miR‐548j binding sites within the Tensin1 3′UTR, as well as sequence alignment of the miR‐548j binding site 1 (WT) and its mutation (Mut) with the miR‐548j targeting sequence. (C) Luciferase reporter assay of HEK293T cells co‐transfected with miR‐548j mimics in a dose dependent manner. (D) Luciferase reporter assay of HEK293T cells co‐transfected with miR‐548j mimics and either WT or Mut luciferase plasmid. *P < 0.05. (E) Western blot analysis of the protein levels of Tensin1 (TNS1) in the indicated cells transfected with NC or miR‐548j mimics. (F) Western blot analysis of Tensin1 protein levels in cells transfected with NC inhibitor or miR‐548j inhibitor.