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. 2016 Feb 23;10(6):838–849. doi: 10.1016/j.molonc.2016.02.002

Figure 5.

Figure 5

miR‐548j and Tensin1 regulate cell invasion via activation of Cdc42. (A) The active form of Cdc42 (Cdc42‐GTP) and total Cdc42 were measured by Western blotting of the indicated cells upon transfection of NC or miR‐548j mimics. (B) The efficiency of the Rho inhibitor ML141 on breast cancer cells with miR‐548j overexpression, represented by the expression level of Cdc42‐GTP. (C) Representative micrographs and quantification of invading cells transfected with a control miRNA or miR‐548j mimics and without or with treatment with either ML141. (D) To confirm the efficiency of siCdc42, total Cdc42 protein levels in the three breast cancer cells overexpressing miR‐548j and transfected with control siRNA (siNC) or Tensin1 siRNA (siTNS1) were analyzed by Western blotting. β‐actin is shown as a loading control. (E) Representative micrographs and quantification of invading cells transfected with a control miRNA or miR‐548j mimics and transfected with a siNC or siCdc42 RNA. Error bars in C and E represent mean ± SEM. *P < 0.05, **P < 0.01.