Skip to main content
. 2016 Jun 7;10(8):1221–1231. doi: 10.1016/j.molonc.2016.05.009

Figure 4.

Figure 4

Comparison of the sensitivity for detection of KRAS mutant cells in whole blood. A. Sanger sequencing. Considering the weak signal, the detection limit is close to 100 cells. As control, no signal was detected in blood from healthy donors or in blood spiked with 50 PANC1 cells. B. TaqMeltPCR: The presence of a mutation melting peak at the correct annealing temperature indicate the presence of KRAS mutations. The sample of healthy donor blood spiked with 50 PANC1 cells is considered at the detection limit when applying validation guidelines. When the assay is performed in “CIVD mode” recommended for clinical applications, the detection limit is 100 cells. C, High Melting Resolution (HRM). The detection limit (red line) is between 75 and 50 cells.