Figure 3.

A) Evaluation of apoptosis by Annexin V-FITC method. The cells were exposed with increasing concentration of brittle star saponin fraction for 24 h by flow cytometry analysis.(a-d) untreated cancer cells, treatment with 12.5, 25 μg/ml brittle star saponin fraction, respectively. B) Apoptotic effect of brittle star saponin fraction on cervical cancer cells estimated by flow cytometry. Flow cytometry histogram of untreated and treated HeLa cells with 12.5, 25μg/ml brittle star saponin exhibited increase in sub-G1 region demonstrating mediation of an apoptotic cell death in cytotoxicity of brittle star saponin