Table 1.
Massspectrometric data and identification of homoisoflavonoids in the chloroform/methanol extract (CME) of O. japonicus root
| Peak no. | t R (min) | [M – H] − m/z | MS/MS m/z (relative intensity, %) | Compound |
|---|---|---|---|---|
| 1 | 14.81 | 373 | 358(18), 183(100), 168(28), 153(24) | 5,2'-Dihydroxy-7,8,4'-trimethoxy-6-methyl homoisoflavanone a |
| 2 | 19.45 | 339 | 324(100), 296(7) | 5-Hydroxy-7,4'-dimethoxy-6,8-dimethyl homoisoflavone a |
| 3 | 23.80 | 373 | 207(100) | 5,7,4'-Trihydroxy-3',5'-dimethoxy-6,8-dimethyl homoisoflavanone a |
| 4 | 27.87 | 343 | 207(100) | 5,7,2'-Trihydroxy-4'-methoxy-6,8-dimethyl homoisoflavanone a |
| 5 | 30.34 | 359 | 344(100), 223(12), 169(94), 154(47) | Ophiopogonanone E a |
| 6 | 31.51 | 357 | 339(41), 222(6), 207(12), 153(100) | 5,7-Dihydroxy-8-methoxy-3',4'-methylene-dioxy-6-methyl homoisoflavanone a |
| 7 | 34.00 | 343 | 325(100), 207(38), 153(69) | 5,7-Dihydroxy-8,4'-dimethoxy-6-methyl homoisoflavanone a |
| 8 | 38.08 | 355 | 340(54), 205(100) | 5-Hydroxy-7-methoxy-3',4'-methylenedioxy-6,8-dimethyl homoisoflavanone a |
| 9 | 39.97 | 355 | 327(32), 218(38), 205(100) | 5,7,2'-Trihydroxy-3',4'-methylenedioxy-6,8-dimethyl homoisoflavone a |
| 10 | 51.31 | 327 | 205(28), 192(100), 164(46) | Ophiopogonanone A a |
| 11 | 54.95 | 313 | 192(100), 164(18) | 5,7-Dihydroxy-4'-methoxy-6-methyl homoisoflavanone a |
| 12 | 61.78 | 339 | 311(100), 218(18), 179(10) | Methylophiopogonone A a |
| 13 | 66.47 | 341 | 206(100), 178(52), 150(3) | Methylophiopogonanone A b |
| 14 | 69.48 | 327 | 206(100), 178(25) | Methylophiopogonanone B b |
| 15 | 84.57 | 353 | 325(100), 297(4) | 6-Aldehydo-isoophiopogonone A a |
| 16 | 87.12 | 355 | 337(23), 327(100), 307(60), 193(32) | 6-Formyl-isoophiopogonanone A a |
| 17 | 89.68 | 339 | 324(27), 311(100), 296(19) | 6-Aldehydo-isoophiopogonone B a |
a
Identification based on HPLC–DAD analysis and MS/MS spectral data.
b
Identification based on the authentic standard.