FIGURE 3.

Comparative analysis of the effects of peroxide-induced oxidative stress on transcription of gene lipL32 in L. Jules and L. Portlandvere (PV) using qRT-PCR. The gene is represented as relative gene abundance (based on log10-transformed gene expression data) with error bars (standard error of mean) for bacteria exposed to hydrogen peroxide at 1 mM/30 min, 10 mM/30 min, 1 mM/60 min, 10 mM/60 min, at the three temperature conditions. Gene expression of 16S rRNA at 30°C (without oxidative stress) served as controls for the purpose of normalization of gene expression in the presence of oxidative stress at the various combinations of exposures, and calculation of fold changes (shown below).