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Bacterial factors are secreted into erythrocytes prior to invasion. F. tularensis Schu S4 was incubated with human erythrocytes for 4 h and processed for immunogold transmission electron microscopy. Ultra-thin sections (70 nm) were probed with anti-F. tularensis rabbit antisera and then a 5 nm gold-labeled anti-rabbit secondary antibody. The black arrows designate the location of F. tularensis antigen within the erythrocyte. Scale bars represent 100 nm.
