Table 1.
Bacterial strains, plasmids, and primers used in this study.
| Strain, plasmid, or primer | Description | Source or reference |
|---|---|---|
| F. tularensis STRAINS | ||
| LVS | F. tularensis subsp. holarctica live vaccine strain | Karen Elkins |
| Schu S4 | F. tularensis subsp. tularensis Schu S4 | BEI Resources |
| ΔmglA | LVS with a deletion of mglA | |
| iglC null | LVS with both copies of iglC deleted | This study |
| iglC null::pTG28 | LVS iglC null trans-complement | This study |
| ΔdotU | LVS with a deletion of dotU | |
| ΔdotU::pDotU | LVS ΔdotU trans-complement | |
| E. coli STRAINS | ||
| DH5α | fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | NEB |
| PLASMIDS | ||
| pJH1 | pMQ225 with the I-SceI restriction site | |
| pTG1 | pJH1 with 1-kb DNA from both the up- and downstream adjacent regions flanking the iglC chromosomal locus | This study |
| pGRP | pFNLTP5 with the FTL_0580 (FGRp) promoter | |
| pGUTS | pGRP with I-SceI under the control of FGRp | |
| pTG28 | pGRP containing iglC under the control of FGRp | This study |
| PRIMERS | ||
| iglC1 | 5′- CATGGCATGCTAAGATTGGTAGTATTGTGGATGTCGAGTCG-3′ | IDT |
| iglC2 | 5′-GTCGACGGTACCACCGGTTTATTATTAACTAGCAGCAGCTGTAGCCG-3′ | IDT |
| iglC3 | 5′-TAATAATAAACCGGTGGTACCGTCGACCTATCTAATTTAGAGTTATATCCAATAAGTGC-3′ | IDT |
| iglC4 | 5′-CATGCTGCAGCTTATCAGTCATTATTTGTAAAGATAACGG-3′ | IDT |
| conf 1F | 5′-CGATCGTAGGGATAACAGGGTAATG-3′ | IDT |
| conf 1R | 5′-CAATTACTTTCTCTCTGATATTCAGAAATAAG-3′ | IDT |
| conf 2F | 5′-CCCGGGGATCCTCTAGAGTCG-3′ | IDT |
| conf 2R | 5′-GTGTATATCGAGAATATCTCATTATATTAAATGTG-3′ | IDT |
IDT, Integrated DNA Technologies.