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. Author manuscript; available in PMC: 2017 May 9.
Published in final edited form as: Cell Rep. 2016 Jan 7;14(3):621–631. doi: 10.1016/j.celrep.2015.12.045

Figure 4. ARTD11cat and ARTD11 WWE Domains are Necessary but Insufficient to Drive ARTD11 Specific MARylation.

Figure 4

(A) Domain architecture of ARTD11, ARTD10, and the chimeric protein (Chimera) created by fusing the ARTD11 n-terminus to the ARTD10cat domain.

(B) Pie chart representing the total MARylated protein targets identified via LC-MS/MS for the chimeric protein. Shared protein targets are indicated by the protein schematics depicted in (A). Shared protein targets were identified based on their presence in at least one of the IG-ARTD10 or IG-ARTD11 LC-MS/MS replicates.

(D) Immunoblot detection of select LC-MS/MS identified ARTD targets (GFP-ARTD, UBE3C, XPO5, NXF1, NUP98, NAGK, WRIP1) following NeutrAvidin enrichment. Overall MARylation levels were determined using streptavidin-HRP (Biotin). ARTD10-specific, ARTD11-specific, ARTD11-WWE dependent, and shared chimera targets are indicated to the left.

See also Figure S3 and Tables S1, S3, and S5.