Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 9;8:743. doi: 10.3389/fpls.2017.00743

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Subburaj, Zhu, Li, Hu and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Phylogenetic tree of the Poaceae cystatins showing relationships between the deduced amino acid sequences of 71 cystatin genes from different plant species. 25 from B. distachyon (BdC), 5 from T. aestivum (WC), 13 from H. vulgare (HvCPI), 1 from S. bicolor (SbC), 3 from Ae. tauschii (AeC), 10 from Z. mays (CC), 12 from O. sativa (OC), 1 from C. lacryma-jobi (CLA) and 1 from S. officinarum (SOF). Multiple alignments of sequences were performed by ClusalW, and the phylogenetic tree was constructed by the neighbor-joining (NJ) method and evaluated by bootstrap analysis. Numbers on the main branches indicate boot strap percentages for 1,000 replicates. The three major groups (1–3) and twelve phylogenetic subgroups (A–L) identified in the plant cystatin family are highlighted with different color arcs and branch, respectively. GenBank numbers for corresponding to the sequences are also shown. Green full circles indicate the BdC genes in the branches.