Table 1.

Potentials (versus NHE) of SQWV reductive sweeps for WT and mutant EndoIII and WT MutY in the presence and absence of DNA.

a Enzyme	b ESQWV (mV), − DNA	b ESQWV (mV), + DNA
WT EndoIII	130 ± 8	c 64 ± 8
WT EndoIII + poly-L glutamate	110 ± 9	-
EndoIII E200K	130 ± 7	-
EndoIII Y205H	125 ± 11	-
EndoIII K208E	141 ± 12	-
WT MutY	d > 130 mV	c 85 ± 3 c, e 80 ± 6

^aWhen used, CNTs were added from a 0.25 mg/mL stock, and all experiments used protein storage buffer as the supporting electrolyte (20 mM sodium phosphate, 0.5 mM EDTA, 150 mM NaCl, pH 7.5 for EndoIII, 20 mM sodium phosphate, 1 mM EDTA, 150 mM NaCl, pH 7.6 for MutY).

^bEach value is the average of 3 or more separate experiments, and error is standard deviation of the mean.

^cNo CNTs present.

^dThe MutY SQWV peaks (Figure 2) were too small and noisy to measure the potential with confidence.

^eOG:FA MutY substrate trap DNA