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. Author manuscript; available in PMC: 2017 May 9.
Published in final edited form as: Cell Rep. 2017 Apr 11;19(2):255–266. doi: 10.1016/j.celrep.2017.03.041

Figure 3. Cellular rhythms of PER2::LUC MEFs treated with FAD.

Figure 3

(A) Representative rhythms of PER2::LUC bioluminescence in MEFs from Per2Luc knockin mice. Cellular rhythms of MEFs were synchronized by 100 nM DEX. Media was replaced by a recording media containing 10–100 μM FAD (or PBS) and 100 μM luciferin for recording of bioluminescence. Period lengths of the bioluminescence rhythms are shown as means ± SEM (n=4, *: p<0.05 by Tukey’s test). (B) NIH3T3 cells were synchronized by 100 nM DEX. Media was replaced by fresh media containing 100 μM FAD (or PBS). Total cell lysates were used for western blotting analysis. Data are shown as means ± SEM (n=3, *:p<0.05 by two-way ANOVA followed by Post-hoc test).