Figure 4.

Schematic diagram of the approach of cancer gene therapy with HDAds. HDAd encoding multiple transgene expression cassettes is intratumorally injected into patient. Suicide gene is driven by cancer cell specific promoter, while other functional molecules are driven by ubiquitous and/or regulatable promoters. Patient will be treated with pro-drug, and cancer cells transduced with HDAd will result in cell death. HDAd vectors transduced in tumor stroma cells (e.g., fibroblast) still remain and express functional molecules in tumor. These functional molecules may activate the infiltrated APCs and enhance the establishment of anti-tumor immunity of host immune system.