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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: J Immunol. 2017 Mar 27;198(9):3404–3409. doi: 10.4049/jimmunol.1602139

Figure 4. Impact of truncating the HMG domain in Tcf1 on T cell development.

Figure 4

(A) Thymic cellularity in WT, Tcf1+/ΔHMG, and Tcf1ΔHMG/ΔHMG mice (n ≥ 4 from 4 experiments). ns, not statistically significant; ***, p<0.001 by Student’s t test.

(B) Thymic maturation stages. Lin− thymocytes were analyzed for DN, DP, CD4+ and CD8+ populations (top panels in B), and the Lin− DN cells were further analyzed for DN1-DN4 subsets (bottom in B). Contour plots are representative from 4 experiments (n ≥ 4).

(C) Detection of Tcf1 by immunoblotting. Cell lysates were extracted from total thymocytes of control, Tcf1+/ΔHMG, and Tcf1ΔHMG/ΔHMG mice, and immunoblotted with anti-Tcf1 or β-actin antibody. Representative data from ≥ 3 experiments are shown.