Table 3. The mutation energy of F1 mutations predicted in silico.

Mutations	Mutation energy /KJ/mol
ASP95>ALA	0.19
PHE96>ALA	0.19
ASP97>ALA	0.27
ILE98>ALA	0.05
SER99>ALA	0.03
PRO100>ALA	0.04
LYS101>ALA	0.15
VAL102>ALA	0.02
ASN103>ALA	0.05
GLY104>ALA	0.93
GLU105>ALA	1.19
ASN106>ALA	0.63
LEU107>ALA	0.57
VAL108>ALA	0.2
GLY109>ALA	0.12
ASP110>ALA	0.68
ASP111>ALA	1.29