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| Mutants | Mutation Energy/ KJ/mol | binding to F1 |
|---|---|---|
| G166W | 7.79 | - |
| G166E | 5.77 | - |
| G167R | 18.48 | - |
| G167E | 12.88 | - |
| G168K | -1.61 | - |
| G168R | -1.14 | unexpressed |
| L169E | 1.5 | - |
| L169D | 1.46 | - |
| L169W | -1.51 | + |
| L169F | -1.14 | + |
| Y170D | 1.2 | - |
| Y170E | 1.03 | - |
| Y170R | -1.57 | + |
| Y214P | 3.93 | - |
| Y214E | 3.11 | - |
| F215P | 1.17 | - |
| G216W | -2.51 | - |
| G216F | -2.3 | - |
| D218R | -1.73 | + |
| D218Y | -1.72 | + |
