Fig 2. Western blot analysis of pro-inflammatory (A) and histone acetylation proteins (B) in study models of retinal degeneration.

(A) Representative western immunoblot was performed for the expression of 11 proteins involved in pro-inflammatory signaling in total retinal protein extracts for normal and mutant retinas at 7 wks (rcd1, xlpra2), 8 wks (erd), 12 wks (erd), and 16 wks (rcd1, xlpra2, xlpra1). The following proteins were analyzed: inflammasome components (CASP1, NLRP3 and PYCARD), inflammasome substrates (IL1B and IL18) and their receptors (IL1R1 and IL18R1), inflammasome receptor (TLR4), common components of IL1B-, IL18- and TLR4-pathways (MYD88 and IRAK4) and macrophages expressing protein (CSF1R). (B) Level of histone acetylation in retinal protein extracts from the same four disease models was evaluated with acetylated-Lysine and acetyl-Histone H3 antibodies at the indicated time points.