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. 2017 May 9;12(5):e0177270. doi: 10.1371/journal.pone.0177270

Fig 4. Assembly of bmAANAT3 complexes with acceptor substrates monitored by NMR.

Fig 4

(A) Mapping of tryptophol induced CSPs to free bmAANAT3. The conserved motifs of the GNAT superfamily are indicated on the left. (B) CSPs as a function of primary sequence for the interaction of tryptophol with free- (blue) and acetyl-CoA-bound bmAANAT3 (red). CSPs greater than the mean or one SD above the mean are marked by continuous and broken lines, respectively. Green, open bars in the free-state mark residues that disappear in the substrate-bound state. (C) Mapping of tryptophol induced CSPs to acetyl-CoA bound bmAANAT3. The bisubstrate, CoA-S-acetyltryptamine (green), was modelled by aligning bmAANAT3 model to the structure of oaAANAT.