Table 1. Dissociation constants measured for bmAANAT3, in μM.
| cofactor | acetyl-CoA | CoA | ||||
| 0.66 ± 0.03 3.70 ± 0.10a |
28 ± 3.0 | |||||
| tryptamine analogs | tryptamine | tryptophol | Tryptophol (acetyl-CoA) | N-acetyltryptamine | N-acetyltryptamine (CoA) | N-acetyltryptamine (acetyl-CoA) |
| 219 ± 8 | 230 ± 12 | 52 ± 2.40 | 11.8 ± 2.7 | 8.9 ± 0.9 | no binding | |
| tyramine analogs | tyramine | tyrosol | tyrosol (acetyl-CoA) | tyrosol (CoA) | tyrosolb(acetyl-CoA) | tyrosolb(CoA) |
| 169 ± 17 | 180 ± 21 | 6.45 ± 0.52 | 52.3 ± 2.5 | 65.7 ± 4.8 | 84.2 ± 12.4 | |
When bmAANAT3 was titrated with an acceptor in the presence of a cofactor (acetyl-Coa or CoA), the cofactor is shown in parenthesis.
a refers to the Kd measured for DAT under identical conditions to bmAANAT3.
b refers to the Kd measured for the bmAANAT3 variant D26A.