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. Author manuscript; available in PMC: 2017 Nov 29.
Published in final edited form as: Biochemistry. 2016 Nov 16;55(47):6524–6534. doi: 10.1021/acs.biochem.6b00714

Figure 3.

Figure 3

LC–MS/MS analysis reveals the formation of disulfide bond between C167 and C344 in the resting enzyme (see Figure 2). High-resolution ESI-MS/MS of trypsin and Asp-N-digested peptides from SQR in the (A) +2 charge state (inset) with the corresponding fragmentation pattern and (B) +4 charge state (inset) with the corresponding fragmentation pattern. y-ions and b-ions are shown for the C344-containing peptide, 339DGYTSCPIVTGYNR352 (red), and the C167-containing peptide, 167CGGAPM(Ox)K173 (cyan).