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. Author manuscript; available in PMC: 2017 May 9.
Published in final edited form as: J Pharm Sci Pharmacol. 2015 Sep;2(3):208–216. doi: 10.1166/jpsp.2015.1069

Figure 2.

Figure 2

dETV1 regulates β-catenin expression in different cell types. (a) dETV1 regulates β-catenin expression in COS-1 cells. COS-1 cells were seeded in six well plates. They were transfected with empty expression vector or expression plasmid pSG5/dETV1 and pSG5/β-catenin as indicated. Cells were transfected with different concentrations of β-catenin and dETV1 (β-catenin: 0.2 μg, Low dETV1: 0.4 μg, High dETV1: 1.4 μg). After 48 hours of transfection, cell lysate was collected and subjected to Western blot analysis. (b) dETV1 regulates β-catenin expression in PNT1A cells. Cells were seeded in six well plates. They were trans-fected with empty expression vector or expression plasmid pSG5/dETV1 and pSG5/β-catenin as indicated. Cells were transfected with different concentrations of β-catenin and dETV1 (β-catenin: 0.2 μg, Low dETV1: 0.4 μg, High dETV1: 1.4 μg). After 48 hours of transfection, cell lysate was collected and subjected to Western blot analysis. (c) dETV1 regulates endogenous β-catenin expression in prostate cancer cells (LNCaP). Cells were seeded in six well plates. They were transfected with empty expression vector or expression plasmid pSG5/HA-dETV1 and pSG5/β-catenin as indicated. Cells were transfected with different concentrations of β-catenin and dETV1 (β-catenin: 0.2 μg, Low dETV1: 0.4 μg, High dETV1: 1.4 μg). After 48 hours of transfection, cell lysate was collected and subjected to Western blot analysis.