Figure 4.

dETV1 stabilizes β-catenin expression. PC-3 cells were seeded and transfected with 0.2 μg of β-catenin (blue line), and with 0.2 μg of β-catenin along with 0.4 μg of dETV1 (red line). After 24 hours of incubation, Cycloheximide, an inhibitor of protein biosynthesis was added. Cell lysates were then collected at 0, 1, 2, 4, and 6 hour time periods and (β-catenin, dETV1, β-actin) protein levels were measured by Western blot followed by densitometry analysis.