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. Author manuscript; available in PMC: 2017 May 9.
Published in final edited form as: J Pharm Sci Pharmacol. 2015 Sep;2(3):208–216. doi: 10.1166/jpsp.2015.1069

Figure 5.

Figure 5

β-catenin expression after PD98059 drug treatment. Prostate cells (PNT1A) were plated in six well plates. They were then transfected with empty expression vector or expression plasmid pSG5/HA-dETV1 and pSG5/β-catenin as indicated. Cells were transfected with different concentrations of β-catenin and dETV1 (β-catenin: 0.2 μg, Low dETV1: 0.6 μg, High dETV1: 1.6 μg). After 24 hours of incubation, cells in lane 2, 4, and 6 were treated with PD98059, a kinase inhibitor and cell lysate was collected and subjected to Western blot analysis. Densitometry analysis was done with Image Gauge software.