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. 2017 May 9;12(5):e0177348. doi: 10.1371/journal.pone.0177348

Fig 7. Authentication of ICP1 And ICP2 cell origin by isoenzyme analysis and PCR-based analysis.

Fig 7

(A) and (B) Analysis of LDH and NP isoenzymes in samples from HeLa (human cells), L929 (mouse cells), DF-1 (chicken cells), ICP1, ICP2 cells and chicken muscle tissue. (C) and (D) Authentication of ICP1 and ICP2 cell lines by PCR based analysis. The same-sized PCR product was produced from ICP1, ICP2, DF-1 cells and chicken muscle tissue using chicken-specific primers, but no PCR product was produced from ICP1 and ICP2 cells using 10 other species-specific primers. (E) The amplified PCR products from the genomic DNA samples from the tested animal species.