Skip to main content
. Author manuscript; available in PMC: 2017 May 9.
Published in final edited form as: Cell Rep. 2017 Apr 11;19(2):307–320. doi: 10.1016/j.celrep.2017.03.044

Figure 5.

Figure 5

GATA3 promotes ATOH1-mediated conversion of SCs to HCs. A diagram of the typical pattern of GATA3 expression in the mature organ of Corti (A). IPC = inner pillar cell, OPC = outer pillar cell. A representative image of GLAST-CreER+;R26tdTomato+ activity in IPh/IB cells one week after Tamox induction at P28 (B). Ectopic ATOH1HA results in the upregulation of POU4F3 (C) and MYO7A (D) in IPh/IB cells (arrows). POU4F3 (E), MYO6 (G), and PVALB (I) are upregulated in PCs & DCs (arrows) of Fgfr3iCreER+;Atoh1HA+;GATA3+ mice. Co-manipulation of GATA3 and ATOH1HA (GATA3+) resulted in significantly more POU4F3+ (F), MYO6+ (H), and PVALB+ (J) SCs than ectopic ATOH1HA alone (GATA3−). Data are presented as mean ± 1 S.E.M, *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars = 20 μm