Table 1.
Antigen | Host | Concentration | Supplier | Catalog# |
---|---|---|---|---|
HA | Rat | 1:100 | Roche | 11 867 431 001 |
MYO6 | Rabbit | 1:200 | Proteus Bioscience | 25–6791 |
MYO7A | Mouse | 1:80 | Santa Cruz | sc-74516 |
PVALB | Mouse | 1:1000 | Sigma | P3088 |
POU4F3 | Rabbit | 1:2500 + TSA | Novus | NBP1–88349 |
Calbindin | Rabbit | 1:500 | Millipore | AB1778 |
Prestin | Goat | 1:500 | Santa Cruz | sc-22692 |
SOX2 | Rabbit | 1:500 | Millipore | AB5603 |
GATA3 | Mouse | 1:200 + AR | BD Biosciences | 558686 |
TCF3 | Goat | 1:100 + AR | Novus | NB100–57092 |
Cleaved caspase 3 | Rabbit | 1:50 | Cell Signaling | 9669 |
KEY: TSA = TSA amplification kit from Invitrogen/Life Technologies
AR = low pH antigen retrieval from Vector Labs (1 hour at 98°C)