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. Author manuscript; available in PMC: 2017 May 9.
Published in final edited form as: Cell Rep. 2017 Apr 11;19(2):307–320. doi: 10.1016/j.celrep.2017.03.044

Table 1.

Primary Antibodies

Antigen Host Concentration Supplier Catalog#
HA Rat 1:100 Roche 11 867 431 001
MYO6 Rabbit 1:200 Proteus Bioscience 25–6791
MYO7A Mouse 1:80 Santa Cruz sc-74516
PVALB Mouse 1:1000 Sigma P3088
POU4F3 Rabbit 1:2500 + TSA Novus NBP1–88349
Calbindin Rabbit 1:500 Millipore AB1778
Prestin Goat 1:500 Santa Cruz sc-22692
SOX2 Rabbit 1:500 Millipore AB5603
GATA3 Mouse 1:200 + AR BD Biosciences 558686
TCF3 Goat 1:100 + AR Novus NB100–57092
Cleaved caspase 3 Rabbit 1:50 Cell Signaling 9669

KEY: TSA = TSA amplification kit from Invitrogen/Life Technologies

AR = low pH antigen retrieval from Vector Labs (1 hour at 98°C)