Figure 2. Effect of naringenin (NG) on the production of MMP-3 in vivo. Naringenin was administered orally for 2 weeks at 20 and 40 mg/kg, beginning 2 days post-induction of osteoarthritis by 2 mg of monosodium iodoacetate (MIA). Total protein was isolated from rat knee articular cartilage. MMP-3 concentration was estimated using western blot densitometry. A, Western blot of MMP-3 and GADPH; B, relative intensity of bands. Data are reported as means±SEM. *P≤0.05, compared to negative control (PBS); ^#P≤0.05 and ^{# #}P≤0.01, compared to MIA-treated group (ANOVA with Bonferonni’s post hoc multiple corrections).