Figure 5. Effect of naringenin on MMP-3 secretion in rat chondrocytes. Primary cultured articular chondrocytes were pretreated with 20 and 40 μM of naringenin. MMP-3 concentration was estimated in the total protein of articular chondrocytes using western blot densitometry. A, Western blot of MMP-3 and GADPH; (B) relative intensity of bands. Data are reported as means±SEM. *P≤0.05, compared to negative control (PBS); ^#P≤0.05 and ^#P≤0.01, compared IL-1β-treated culture (ANOVA with Bonferonni’s post hoc multiple corrections).