Figure 1. Effects of cold-inducible RNA-binding protein (CIRBP) on cell viability of H9C2 cells with myocardial ischemia. CIRBP mRNA expression levels in cells transfected with pcDNA3.1 or pc-CIRBP (A) and siNC or siCIRBP (B) were assessed by quantitative reverse transcription PCR. CIRBP protein expression levels in cells transfected with pcDNA3.1 or pc-CIRBP (C) and siNC or siCIRBP (D) were assessed by western blot analysis. (E), Cell viability of transfected cells. Cells were transfected with pcDNA3.1, pc-CIRBP, siNC or siCIRBP and cultured in serum-free medium with hypoxia. Cell viability was determined by AlamarBlue assay. pc-CIRBP: pcDNA3.1 containing CIRBP coding sequence; siCIRBP: CIRBP-specific small interfering RNA; siNC: siCIRBP negative control. Data are reported as means±SD of 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001 (ANOVA).